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KMID : 0861020140290060125
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Korea Journal of Herbology
2014 Volume.29 No. 6 p.125 ~ p.132
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The Study of Anti-inflammatory Effect of Suryeon-hwan Water Extract in RAW 264.7 Cells
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Yoon Yeo-Hwan
Kim Sung-Bae
Kang Ok-Hwa
Mun Su-Hyun
Seo Yun-Soo
Yang Da-Wun
Kang Da-Hye
Wi Gyeong
Lim Jae-Soo
Kim Ma-Ryong
Kwak Nam-Won
Kong Ryong
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Abstract
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Objectives : Suryeon-hwan (SRH) exhibits potent anti-inflammatory activity with an unknown mechanism. However, there has been a lack of studies regarding the effects of SRH on the inflammatory activities and effector inflammatory disease mechanism about macrophage before is not known. So, the investigation focused on whether SRH inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) productions, as well as the expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and mitogen-activated protein kinases (MAPKs) in LPS-stimulated RAW 264.7 cells.
Methods : Cells were treated with 200 ng/mL of LPS 30 min prior to the addition of SRH. Cell viability was measured by MTS assay. The production of nitric oxide (NO) was determined by reacting cultured medium with Griess reagent. The content of level of cytokines (PGE, IL-6) in media from LPS-stimulated Raw 264.7 cells was analyed by ELISA kit. The expression of COX-2, iNOS and MAPKs was investigated by Western blot, RT-PCR.
Results : We found that SRH inhibited LPS-induced NO, PGE2 and IL-6 productions as well as the expressions of iNOS and COX-2. Furthermore, SRH suppressed the LPS-induced phosphorylation of MAPK and extracellular signal-regulated kinase 1/2 (ERK 1/2) activation.
Conclusions : These results suggest that SRH has inhibitory effects on LPS-induced PGE2, NO, and IL-6 production, as well as the expressions of iNOS and COX-2 in the murine macrophage. These inhibitory effects occur through blockades on the phosphorylation of MAPKs following activation.
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KEYWORD
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Suryeon-hwan, SRH , anti-inflammatory activity, RAW 264.7 cells, mitogen-activated protein kinases, MAPKs
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